Further Information
JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3
WB: 0.25-4 μg/mL; ICC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
JMJD3 Antibody: The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.
- Agger et al. Nature 2007; 449:731-4.
- De Santa et al. Cell 2007; 130:1083-94.
- Sola et al. PLoS One 2011; 6:e18421.
JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Anti-JMJD3 antibody (6657) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3.
The immunogen is located within amino acids 1370-1420 of JMJD3.
Human JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD). Mouse JMJD3 has one isoform (1641aa, 176kD) and rat JMJD3 also has one isoform (1356aa, 146kD). 6657 can detect human, mouse and rat.
23135
lysine (K)-specific demethylase 6B
KDM6B
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 180
Observed: 180 kD
EAW90126
122937251
JMJD3 Antibody is affinity chromatography purified via peptide column.
Stem Cell
This antibody is specific for JMJD3 and will not recognize other JMJD proteins.
O15054
Optimal dilutions for each application to be determined by the researcher.
Independent Antibody Validation in Cell lines (Figure 2) shows similar JMJD3 expression profile in human, mouse and rat cell lines detected by two independent anti-IFN-beta antibodies that recognize different epitopes, 6657 against C-terminus domain and 14-618 against N-terminus domain. IFN-beta proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.