Further Information
CASKIN2 Antibody: ANKS5B, KIAA1139, Caskin-2, CASK-interacting protein 2
CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
CASKIN2 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting protein ) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN2 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. It is expressed ubiquitously with highest levels present in fetal and adult liver tissue.
- Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13
- Weng YL, Liu N, DiAntonio A, et al. The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance. Neurosci. 2011; 31:4421-33.
- Tabuchi K, Biederer T, Butz S, et al. CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with CASKIN2, a novel CASK-binding protein. J. Neurosci. 2002; 22:4264-73.
CASKIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2.
The immunogen is located within amino acids 380 - 430 of CASKIN2.
57513
CASK interacting protein 2
CASKIN2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 123, 132 kDa
Observed: 120 kDa
NP_065804
217416347
CASKIN2 Antibody is affinity chromatography purified via peptide column.
Signal Transduction
CASKIN2 antibody is predicted to not cross-react with other CASKIN family members. Three isoforms of CASKIN2 are known to exist; this antibody will not detect the shortest isoform.
Q8WXE0
Optimal dilutions for each application to be determined by the researcher.