ProSci

RUSC2 Antibody

Product Code:
 
PSI-6659
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of RUSC2 in SK-N-SH cell lysate with RUSC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
2 / 3
Immunohistochemistry of RUSC2 in human brain tissue with RUSC2 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of RUSC2 in human brain tissue with RUSC2 antibody at 20 μg/mL.
❮❯

Western blot analysis of RUSC2 in SK-N-SH cell lysate with RUSC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of RUSC2 in human brain tissue with RUSC2 antibody at 5 μg/mL.
Immunofluorescence of RUSC2 in human brain tissue with RUSC2 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6659-0.02mg0.02mg£150.00
Quantity:
PSI-6659-0.1mg0.1mg£449.00
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Further Information

Additional Names:
RUSC2 Antibody: Iporin, KIAA0375, Iporin, Interacting protein of Rab1
Application Note:
RUSC2 antibody can be used for detection of RUSC2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RUSC2 Antibody: RUSC2, also known as Iporin, shares with the related protein RUSC1 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC2 is a rab1-interacting protein that also interacts with GM130, another rab1-interacting protein. RUSC2 interacts with specific rab1 isoforms with different rab-binding specificity. It has been suggested that RUSC2 may function as a link between the targeting of ER derived vesicles triggered by the rab1 GTPase and a signaling pathway composed of proteins containing SH3 and/or poly-proline regions.
Background References:
  • Bayer M, Fischer J, Kremerskthen J, et al. Identification and characterization of Iporin as a novel interaction partner for rab1. BMC Cell Biol. 2005; 29:6:15.
  • Katoh M and Katoh M. Characterization of RUSC1 and RUSC2 genes in silico. Oncol. Rep. 2004; 12:933-8
  • Fukuda M, Kobayashi H, Ishibashi K, et al. Genome-wide investigation of the rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 2011; 36:155-70
Buffer:
RUSC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RUSC2 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human RUSC2.

The immunogen is located within amino acids 70 - 120 of RUSC2.
NCBI Gene ID #:
9853
NCBI Official Name:
RUN and SH3 domain containing 2
NCBI Official Symbol:
RUSC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055621
Protein GI Number:
55741719
Purification:
RUSC2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least three isoforms are known to exist; this antibody will detect all three isoforms. This antibody is predicted to not cross-react with RUSC1.
Swissprot #:
Q8N2Y8
User NOte:
Optimal dilutions for each application to be determined by the researcher.