ProSci

SEPT1 Antibody

Product Code:
 
PSI-6903
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of SEPT1 in Raji cell lysate with SEPT1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunocytochemistry of SEPT1 in Raji cells with SEPT1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of SEPT1 in Raji cells with SEPT1 antibody at 20 μg/mL.
❮❯

Western blot analysis of SEPT1 in Raji cell lysate with SEPT1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SEPT1 in Raji cells with SEPT1 antibody at 5 μg/mL.
Immunofluorescence of SEPT1 in Raji cells with SEPT1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6903-0.02mg0.02mg£150.00
Quantity:
PSI-6903-0.1mg0.1mg£449.00
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Further Information

Additional Names:
SEPT1 Antibody: LARP, SEP1, DIFF6, PNUTL3, Septin-1, LARP
Application Note:
SEPT1 antibody can be used for detection of SEPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SEPT1 Antibody: SEPT1 (also known as Septin 1), is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. SEPT1 was initially identified through yeast two-hybrid screening as an interaction partner for the serine/threonine kinase Aurora-B. During mitosis, SEPT1 localizes to the spindle pole, suggesting that it may play a role in cytokinesis and chromosome congression.
Background References:
  • Qi M, Yu W, Liu S, et al. Septin1, a new interaction partner for human serine/threonine kinase aurora-B. Biochem. Biophys. Res. Commun. 2005; 336:994-1000.
  • Russell SE and Hall PA. Septin genomics: a road less travelled. Biol. Chem. 2011; 392:763-7.
  • Zhu J, Qi ST, Wang YP, et al. Septin1 is required for spindle assembly and chromosome congression in mouse oocytes. Dev. Dyn. 2011; 240:2281-9.
Buffer:
SEPT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SEPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SEPT1.

The immunogen is located within amino acids 300 - 350 of SEPT1.
NCBI Gene ID #:
1731
NCBI Official Name:
septin 1
NCBI Official Symbol:
Sep-01
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_443070
Protein GI Number:
431822382
Purification:
SEPT1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least two isoforms of SEPT1 are known to exist. This antibody is predicted to not cross-react with other Septin protein family members.
Swissprot #:
Q8WYJ6
User NOte:
Optimal dilutions for each application to be determined by the researcher.