Further Information
BRUCE Antibody: BRUCE, APOLLON, KIAA1289, Baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE
BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination.
- Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
- Chen Z, Naito M, Hori S, et al. A human IAP-gamily gene, apollon, expressed in human brain cancer cells. Biochem. Biophys. Res. Commun. 1999; 264:847-54
- Hao Y, Sekine K, Kawabata A, et al. Apollon ubiquitinates SMAC and Caspase-9, and has an essential cytoprotection function. Nat. Cell Biol. 2004; 6:849-60.
BRUCE Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE.
The immunogen is located within amino acids 4760 - 4810 of BRUCE.
57448
baculoviral IAP repeat-containing protein 6
BIRC6
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
534 kDa
NP_057336
153792694
BRUCE Antibody is affinity chromatography purified via peptide column.
Apoptosis
BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist.
Q9NR09
Optimal dilutions for each application to be determined by the researcher.