Further Information
ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
- Pakdel F, Reese JC, and Katzenellenbogen BS. Identification of charged residues in an N- terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 1993; 7:1408-17.
- Sheeler CQ, Singleton DW, and Khan SA. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 2003; 29:237-55.
- Rogatsky I, Trowbridge JM, and Garabedian MJ. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 1999; 274:22296-302.
ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Horse: (100%)
ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1.
The immunogen is located within amino acids 250 - 300 of ESR1.
2099
estrogen receptor 1
ESR1
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 65 kDa
Observed: 62 kDa
NP_001116214
170295804
ESR1 Antibody is affinity chromatography purified via peptide column.
Signal Transduction
ESR1 antibody is human, mouse and rat reactive.
P03372
Optimal dilutions for each application to be determined by the researcher.