Further Information
DDC Antibody : AADC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase
DDC antibody can be used for detection of DDC by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
DOPA decarboxylase (DDC) belongs to the group II decarboxylase family of proteins (1). It is an important protein in the catecholamine biosynthesis pathway. DDC catalyzes the second reaction in the biosynthesis of catecholamines, trace amines and serotonin (1,2). It can form a homodimer and is expressed in the central nervous system (2). DDC can be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease, neuroendocrine tumors and drug abuse (3,4). Defects in DDC gene may cause the autosomal recessive disorder AADC deficiency (4).
- Berry MD, Juorio AV, Li XM, et al. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem. Res. 1996; 21:1075-87.
- Sumi-Ichinose C, Ichinose H, Takahashi E, et al. Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and Serotonin biosynthesis. Biochemistry 1992; 31:2229-38.
- Haycock JW, Becker L, Ang L, et al. Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J. Neurochem. 2003; 87:574-85.
- Chang YT, Sharma R, Marsh JL, et al. Levodopa-responsive aromatic L-amino acid decarboxylase deficiency. Ann. Neurol. 2004; 55:435-8.
DDC Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (92%), Guinea pig: (77%), Pig: (77%)
DDC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DDC.
The immunogen is located within amino acids 390 - 440 of DDC .
1644
dopa decarboxylase (aromatic L-amino acid decarboxylase)
DDC
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 53 kDa
Observed: 53 kDa
NP_000781
4503281
DDC Antibody is affinity chromatography purified via peptide column.
Neuroscience
DDC antibody is human, mouse and rat reactive.
P20711
Optimal dilutions for each application to be determined by the researcher.