ProSci

FOXO3A Antibody

Product Code:
 
PSI-45-054
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-054 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Detected by chemiluminescence.
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45-054 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Detected by chemiluminescence.

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CodeSizePrice
PSI-45-054-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
FOXO3, FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog, FOXO2, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, forkhead box O3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Heart lysates (calculated MW of 71.3kDa according to NP_001446.1). Recommended concentration: 0.1-1 ug/ml. Primary incubation was 1 hour.
Background References:
  • Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Fusion with MLL in leukemia Cell. 1999 Mar 19;96(6):857-68.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-GAKQASSQSWVPG
NCBI Gene ID #:
2309
NCBI Official Name:
forkhead box O3
NCBI Official Symbol:
FOXO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001446.1
Protein GI Number:
4503739
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Both variants represent identical protein (NP_001446.1 and NP_963853.1).
Swissprot #:
O43524