Further Information
FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot:Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Negative Control: Human Pancreas lysate.
Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
- Chatila TA, Blaeser F, Ho N, Lederman HM, Voulgaropoulos C, Helms C, Bowcock AM. JM2, encoding a fork head-related protein, is mutated in X-linked autoimmunity-allergic disregulation syndrome. J Clin Invest. 2000 Dec;106(12):R75-81.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
The immunogen for this antibody is: SQRPSRCSNPTPGP
50943
forkhead box P3
FOXP3
Homo sapiens
Liquid
NP_054728.2, NP_001107849.1
31982943
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction
This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
Q9BZS1