Further Information
GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell lines Hek293 and HeLa and in Human Liver, Tonsil, Mouse Spleen, and Rat Brain and Spleen lysates (calculated MW of 36.1kDa according to Human NP_002037.2 and 35.8kDa according to Mouse NP_032110.1 and Rat NP_058704.1). Recommended concentration: 0.01-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2.5ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of HeLa cells. Recommended concentration: 10ug/ml.
- Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM,
Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nat Med. 1996 Mar;2(3):347-50.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Pig
The immunogen for this antibody is: C-HQVVSSDFNSDT
2597
glyceraldehyde-3-phosphate dehydrogenase
GAPDH
Homo sapiens
Liquid
NP_002037.2
7669492
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis ReagentsControl Load Control
GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
P04406