Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
GJC3, gap junction protein, gamma 3, 30.2kDa, CX30.2, CX31.3, Cx29, GJE1, connexin 29, connexin 30.2, connexin 31.3, gap junction protein, epsilon 1, 29kDa
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Brain (Cerebellum) and in Human Skeletal Muscle lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.3kDa according to NP_853516.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- Sargiannidou I, Ahn M, Enriquez AD, Peinado A, Reynolds R, Abrams C, Scherer SS, Kleopa KA, Human oligodendrocytes express Cx31.3: function and interactions with Cx32 mutants. Neurobiology of disease 2008 May 30 (2): 221-33.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Human, Dog
The immunogen for this antibody is: C-LTSESTRRHKKATDS
349149
gap junction protein, gamma 3, 30.2kDa
GJC3
Homo sapiens
Liquid
NP_853516.1
31559821
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction
Q8NFK1