Product Code:
PSI-XP-5286
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
TNF-a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
1 / 2
2 / 2
❮❯No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Show All
Further Information
Tnfa, RATTNF, TNF-alpha, Tnfa, Tnfsf2, Tumor necrosis factor, Cachectin, TNF-a
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF alpha (2.0 ng/mL), a concentration of 0.145 - 0.165 μg/mL of this antibody is required.
ELISA:
To detect Rat TNF alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat TNF alpha.
Sandwich:
To detect Rat TNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat TNF-α (XP-5286Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-α.
Western Blot:
To detect Rat TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.
batch dependent
Unconjugated
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat TNF alpha.
24835
tumor necrosis factor
Tnf
Rattus norvegicustus norvegicus
Lyophilized
P16599
135938
Anti-Rat TNF alpha specific antibody was purified by affinity chromatography employing immobilized Rat TNF alpha matrix.
Immunology,Chemokines & Cytokines,Signal Transduction,Growth Factor,Antibody Pairs, Functional Assays
P16599
Centrifuge vial prior to opening.