ProSci

LAT Antibody

Product Code:
 
PSI-45-080
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-080 (0.01ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-080 (0.01ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-080-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
LAT1, LAT, linker for activation of T cells, pp36, 36 kDa phospho-tyrosine adaptor protein, linker for activation of T-cells family member 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 27.9kDa according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03ug/ml.
Background References:
  • Finco TS, Kadlecek T, Zhang W, Samelson LE, Weiss A. LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway. Immunity. 1998 Nov;9(5):617-26.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-GAPDYENLQELN
NCBI Gene ID #:
27040
NCBI Official Name:
linker for activation of T cells
NCBI Official Symbol:
LAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1
Protein GI Number:
7657293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).
Swissprot #:
O43561