Further Information
POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3, POU class 5 homeobox 1, DADB-104B20.2, OCT4, POU domain, class 5, transcription factor 1
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30kDa band observed in Human Placenta, and approx 28kDa in Mouse Heart lysates (calculated MW of 30.1kDa according to Human NP_976034.3 and 25.0kDa according to Mouse NP_001239381.1). Recommended concentration: 0.5--2ug/ml. Primary incubation was 1 hour
- Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L. OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma. Am J Surg Pathol. 2004 Jul;28(7):935-40.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Dog
The immunogen for this antibody is: C-VTTLGSPMHSN
5460
POU class 5 homeobox 1
POU5F1
Homo sapiens
Liquid
NP_002692.2, NP_976034.3
42560248
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Q01860