Product Code:
PSI-XP-5231
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MIG antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
CMK, MIG, Humig, SCYB9, crg-10, CMK, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, HuMIG
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.
ELISA:
To detect hMIG by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIG.
Sandwich:
To detect hMIG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIG (XP-5231Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.
Western Blot:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
MIG, a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor.
batch dependent
Unconjugated
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG (human MIG).
4283
chemokine (C-X-C motif) ligand 9
CXCL9
Homo sapiens
Lyophilized
Q07325
585487
Anti-hMIG specific antibody was purified by affinity chromatography employing immobilized hMIG matrix.
Chemokines & Cytokines,Signal Transduction,Homeostasis,Antibody Pairs, Functional Assays
Q07325
Centrifuge vial prior to opening.