Further Information
HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A, OTTHUMP00000016223, OTTHUMP00000016224
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b( (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1). The 50kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4ug/ml.
- Chiappetta G, Botti G, Monaco M, Pasquinelli R, Pentimalli F, Di Bonito M,
D'Aiuto G, Fedele M, Iuliano R, Palmieri EA, Pierantoni GM, Giancotti V, Fusco
A. HMGA1 protein overexpression in human breast carcinomas: correlation with ErbB2
expression. Clin Cancer Res. 2004 Nov 15;10(22):7637-44
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
The immunogen for this antibody is: C-LASKQEKDGTEK
3159
high mobility group AT-hook 1
HMGA1
Homo sapiens
Liquid
NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1
22208967
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1).
P17096