Further Information
PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4kDa according to NP_058644.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10ug/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10ug/ml.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
- Johansson A, Driessens M, Aspenstrom P. The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1. J Cell Sci. 2000 Sep;113 ( Pt 18):3267-75.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
The immunogen for this antibody is: C-GSRIRGDGSGFSL
50855
par-6 family cell polarity regulator alpha
PARD6A
Homo sapiens
Liquid
NP_058644.1, NP_001032358.1
8394417
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).
Q9NPB6