Further Information
DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot:Approx 150kDa band observed in Human Cerebellum lysates (calculated MW of 142kDa according to NP_004073.2). Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.
Immunohistochemistry:In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4ug/ml. A customer found positive results in IHC on Human HeLa cells.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10ug/ml.
Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Holzbaur EL, Tokito MK. Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization. Genomics. 1996 Feb 1;31(3):398-9. No abstract available.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
The immunogen for this antibody is: C-QEQLHQLHSRLIS
1639
dynactin 1
DCTN1
Homo sapiens
Liquid
NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1, NP_001177765.1, NP_001177766.1
13259510
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise all reported isoforms.
Q14203