Further Information
ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 34-35kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3kDa according to NP_005722.1). Recommended concentration: 0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5ug/ml.Immunofluorescence: :Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.
- Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997 Jul 28;138(2):375-84.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: C-EMKTITGKTFSSR
10109
actin related protein 2/3 complex, subunit 2, 34kDa
ARPC2
Homo sapiens
Liquid
NP_005722.1, NP_690601.1
5031599
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis
The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
O15144