Further Information
CAPZB, capping protein (actin filament) muscle Z-line, beta, CAPB, CAPZ, CAPPB, Cap Z, RP4-657E11.7, MGC104401, MGC129749, MGC129750, Cap Z, F-actin capping protein beta subunit
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in lysates of cell lines Jurkat, HeLa and NIH-3T3 and approx 28kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 30.6kDa according to Human NP_004921.1 and 31.3kDa according to Mouse NP_001032850.1, and 30.6kDa according to Rat NP_001005903.1 and Pig NP_001106915.1]. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Wang CY et al, J Biomed Sci. 2011 Feb 7;18:13. PMID: 21299884.
- Hart MC, Cooper JA. Vertebrate isoforms of actin capping protein beta have distinct functions In vivo.gelsolin/severin and profilin protein families. J Cell Biol 1999 Dec 13;147(6):1287-98
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Cow
The immunogen for this antibody is: NDLVEALKRKQQC
832
capping protein (actin filament) muscle Z-line, beta
CAPZB
Homo sapiens
Liquid
NP_004921.1
4826659
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to be specific for the beta2 isoform of capping protein, found in non-muscle cells and should not recognise the beta1 form.
P47756