ProSci

cdc25A Antibody

Product Code:
 
PSI-79-169
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).
❮❯

Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).

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PSI-79-169-0.1mg0.1mg£488.00
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Further Information

Additional Names:
CDC25A2, MEK1, PRKMK1, CDC25M3, MPIP1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Background References:
  • Jin J, et al. (2003) Genes 17 (24): 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14): 3694-703.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.
Modifications:
Phospho-Specific
NCBI Gene ID #:
993
NCBI Official Name:
cell division cycle 25A
NCBI Official Symbol:
CDC25A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
NP_001780.2
Protein GI Number:
42490758
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
Swissprot #:
P30304