ProSci

Centromere Protein F Antibody

Product Code:
 
PSI-45-393
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-393 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-393 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-45-393-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/400ka (mitosin), RP11-262H5.1, hcp-1, centromere protein F, mitosin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Background References:
  • Liao H, Winkfein RJ, Mack G, Rattner JB, Yen TJ. CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. J Cell Biol. 1995 Aug;130(3):507-18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: GLESKGSENCKVQ
NCBI Gene ID #:
1063
NCBI Official Name:
centromere protein F, 350/400kDa
NCBI Official Symbol:
CENPF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAA82889.1, AAA82935.1, AAA86889.1
Protein GI Number:
55770834
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
The immunizing peptide shows one mismatch with the corresponding sequence of the NCBI-curated isoform NP_057427.3.
Swissprot #:
P49454