Further Information
CXCR3, chemokine (C-X-C motif) receptor 3, HGNC:4540, CD183, CKR-L2, CMKAR3, GPR9, IP10, IP10-R, Mig-R, MigR, G protein-coupled receptor 9, IP10 receptor, Mig receptor, chemokine (C-X-C) receptor 3
Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot:Preliminary experiments showed a band at approx 55-60kDa in Human Tonsil and Mouse Spleen lysates after 1ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note we cannot currently find an explanation in the literature for this band, given the calculated size of 45.5kDa according to NP_001136269.1
Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
- Martini G, Zulian F, Calabrese F, Bortoli M, Facco M, Cabrelle A, Valente M, Zacchello F, Agostini C. CXCR3/CXCL10 expression in the synovium of children with juvenile idiopathic arthritis. Arthritis Res Ther. 2005;7(2):R241-9. Epub 2005 Jan 7.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat
The immunogen for this antibody is: C-RRDSSWSETSEA
2833
chemokine (C-X-C motif) receptor 3
CXCR3
Homo sapiens
Liquid
NP_001495.1, NP_001136269.1
4504099
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
P49682