ProSci

CYP3A4 Antibody

Product Code:
 
PSI-45-448
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-448 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
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45-448 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

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PSI-45-448-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CYP3A4, cytochrome P450, family 3, subfamily A, polypeptide 4 , HGNC:2637, CP33, CP34, CYP3A, CYP3A3, HLP, NF-25, P450C3, P450PCN1, P450-III, steroid inducible, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, cytochrome P450, subfamily IIIA, polypeptide 4, glucocorticoid-inducible P450, nifedipine oxidase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Background References:
  • Choi MH, Skipper PL, Wishnok JS, Tannenbaum SR. Characterization of testosterone 11{beta}-hydroxylation catalyzed by human-liver microsomal cytochromes P450. Drug Metab Dispos. 2005 Mar 11;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KESRLEDTQKHR
NCBI Gene ID #:
1576
NCBI Official Name:
cytochrome P450, family 3, subfamily A, polypeptide 4
NCBI Official Symbol:
CYP3A4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_059488.2, NP_001189784.1
Protein GI Number:
13435386
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_059488.2; NP_001189784.1) and it is not expected to cross-react with 3A5, 3A7 ot 3A43.
Swissprot #:
P08684