ProSci

DEAD-box protein 6 Antibody

Product Code:
 
PSI-45-471
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-471 (0.3ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-471 (0.3ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-471-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD),
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Thymus. Recommended concentration: 5ug/ml.
Background References:
  • Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol. 2004 Apr;165(1):31-40. Epub 2004 Apr 5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-AEYHSEPVEDEKP
NCBI Gene ID #:
1656
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 6
NCBI Official Symbol:
DDX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004388.1
Protein GI Number:
4758140
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P26196