ProSci

YB1 (phospho Ser102) Antibody

Product Code:
 
PSI-79-928
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20°C for up to one year.
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Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody. The lane on the right is treated with the antigen-specific peptide.

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PSI-79-928-0.1mg0.1mg£488.00
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Further Information

Additional Names:
CBF-A, NSEP1, EFI-A, MSY-1, YBX1
Application Note:
Western Blot: 1:500~1:1000
Background:
YB-1 is a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP.
Background References:
  • Sakura H., Gene 73:499-507(1988).
  • Didier D.K. Proc. Natl. Acad. Sci. U.S.A. 85:7322-7326(1988).
  • Kolluri R., Nucleic Acids Res. 19:4771-4771(1991).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
YB1 (phospho Ser102) antibody was raised against a peptide sequence around phosphorylation site of Serine 102 (L-R-S (p)-V-G) derived from Human YB1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4904
NCBI Official Name:
Y box binding protein 1
NCBI Official Symbol:
YBX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
NP_004550.2
Protein GI Number:
34098946
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.
Swissprot #:
P67809