ProSci

CD80 Recombinant Protein

Product Code:
 
PSI-96-044
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-044-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
B7-1 and B7-2, together with their receptors CD28 and CTLA?4, constitute one of the dominant co-stimulatory pathways that regulate T? and B?cell responses. Although both CTLA?4 and CD28 can bind to the same ligands, CTLA?4 binds to B7?1 and B7?2 with a 20 ? 100 fold higher affinity than CD28 and is involved in the down?regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Background References:
  • Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181?7.
  • Stamper CC., et al., 2001, Nature (England) 410 (6828): 608?11.
  • Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
  • Khan N., et al., 2007, Int. Immunol. 19:477-486.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, His Tag at 20ug/mL (100 ul/well),can bind Human CD28, Fc Tag with a linear of 0.2-20 ug/mL. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The?ED50 for?this?effect?is?typically?0.01 - 0.1?ug/mL?in?the?presence?of?PHA.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
941
NCBI Official Name:
CD80 molecule
NCBI Official Symbol:
CD80
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
25.8 kDa
Protein Accession #:
P33681-1
Purity:
>90% as determined by SDS-PAGE.

Less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P33681

Documents