Further Information
PECAM1, CD31, FLJ34100, FLJ58394
This recombinant protein can be used for WB. For research use only.
Cluster of Differentiation 31 (CD31) is also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.
- Newman PJ, Newman DK., 2004, Arterioscler. Thromb. Vasc. Biol. 23 (6): 953?64.
- Kalinowska A, Losy J., 2007, Eur. J. Neurol. 13 (12): 1284?90.
- Almendro N, et al., 2007, J. Immunol. 157 (12): 5411?21.
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 50000 cells/well are added to rhCD31 coated plates (3. 3 μg/mL, 100 μl/well) in the presence of 20 ng/mL PMA, approximately 55 - 65% will adhere after 30 minutes at 37˚C.
50 mM Tris, 150 mM NaCl, pH7.5
His Tag
5175
platelet/endothelial cell adhesion molecule 1
PECAM1
Homo sapiens
Lyophilized
Predicted Molecular Weight:
66.4 kDa
P16284-1
>95% as determined by SDS-PAGE.
Less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
HEK293 cells
P16284