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2',7'-Dichlorofluorescein

Product Code:
 
CDX-D0004
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
+4°C
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Chemical Structure
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Chemical Structure

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CDX-D0004-G01010 g£108.00
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CDX-D0004-G02525 g£205.00
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CDX-D0004-G100100 g£609.00
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Further Information

Alternate Names/Synonyms:
Dichlorofluorescein; DCF
Appearance:
Beige powder.
CAS:
76-54-0
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Keep cool and dry.Protect from light and moisture.
Hazards:
H315, H319, H335
InChi:
InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChiKey:
VFNKZQNIXUFLBC-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 76-54-0. Formula: C20H10Cl2O5. MW: 401.2. Synthetic Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).
MDL:
MFCD00005047
Molecular Formula:
C20H10Cl2O5
Molecular Weight:
401.2
Package Type:
Vial
Precautions:
P261, P305, P351, P338
Product Description:
Fluorescent dye. Spectral data: Extinction 504nm/Emmission 529nm, in 0.1 M Tris pH 8.0. Used for the determination of carbohydrates by fluorescence densitometry after TLC, in the determination of H2O2 by chemiluminescence, and as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. Used as a component/substrate of the 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS).
Purity:
>90% (T)
Signal word:
Warning
SMILES:
OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(O)C=C1O2
Solubility Chemicals:
Soluble in ethanol (25 mg/ml). Sligthly solubel in water (0.1mg/ml), DMSO.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) M.L. Baumann & A.L. Hatmaker; Clin. Chem. 7, 356 (1961) | (2) G. G?bitz, et al.; J. Chromatogr. 117, 337, (1976) | (3) H. Watanabe, et al.; Anal. Sci. 2, 461, (1986) | (4) W.D. Pfeffer & E.S. Yeung; J. Chromatogr. 506, 401, (1990) | (5) H. Zhu, et al.; Arch. Toxicol. 68, 582 (1994)