5(6)-FAM N-succinimidyl ester
Product Code:
CDX-C0011
CDX-C0011
Regulatory Status:
RUO
RUO
Shipping:
Ambient
Ambient
Storage:
+4°C
+4°C
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Code | Size | Price |
---|
CDX-C0011-M100 | 100 mg | £126.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
- Further Information
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Further Information
Alternate Names/Synonyms:
5(6)-Carboxyfluorescein NHS ester (mixture of isomers); 5(6)-CFSE; 5(6)-FAM SE; FLUOS
Appearance:
Yellow to orange powder.
CAS:
117548-22-8
EClass:
32160000
Form (Short):
solid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C20H12O5.C6H7NO4/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;1-4(8)11-7-5(9)2-3-6(7)10/h1-10,21H,(H,23,24);2-3H2,1H3
InChiKey:
STUDUJDWFUAOEN-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 117548-22-8. Formula: C25H15NO9. MW: 473.4. Synthetic. Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492; lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0.
MDL:
MFCD04119713
Molecular Formula:
C25H15NO9
Molecular Weight:
473.4
Package Type:
Vial
Product Description:
Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492; lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0.
Purity:
>98% (HPLC)
SMILES:
OC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=O)=C1.CC(ON5C(CCC5=O)=O)=O
Solubility Chemicals:
Soluble in DMSO or dimethylformamide.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
Documents
References
(1) P.R. Banks & D.M. Paquette; Bioconjug. Chem. 6, 447 (1995) | (2) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996) | (3) D. Fulcher & S. Wong; Immunol. Cell Biol. 77, 559 (1999) | (4) T. Sathiyaseelan & C.L. Baldwin; Res. Vet. Sci. 69, 275 (2000) | (5) I. Tsuge, et al.; Allergol. Int. 56, 149 (2007)