Biotin-(5-fluorescein)-conjugate
Product Code:
CDX-B0078
CDX-B0078
Regulatory Status:
RUO
RUO
Shipping:
Ambient
Ambient
Storage:
Short Term: +4°C. Long Term: -20°C
Short Term: +4°C. Long Term: -20°C
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
CDX-B0078-M001 | 1 mg | £84.00 |
Quantity:
CDX-B0078-M005 | 5 mg | £304.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
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Further Information
Alternate Names/Synonyms:
Biotin-4-Fluorescein; B4F; N-(Biotinylamidoethyl)-fluorescein-5-carboxamide; N-(Fluorescein-5-carbamidoethyl)biotinamide
Appearance:
Yellow solid.
CAS:
957494-27-8
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1
InChiKey:
DFUFXKZUEOKPSD-LFERIPGTSA-N
Long Description:
Chemical. CAS: 957494-27-8. Formula: C33H32N4O8S. MW: 644.7. Synthetic. Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.
MDL:
MFCD03791084
Molecular Formula:
C33H32N4O8S
Molecular Weight:
644.7
Package Type:
Vial
Product Description:
Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.
Purity:
>90% (HPLC)
SMILES:
O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
Documents
References
(1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999) | (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999) | (3) N. Humbert, et al.; Electrophoresis 26, 47 (2005) | (4) F.M. Aslan, et al.; PNAS 102, 8507 (2005) | (5) M.J. Waner & D.P. Mascotti; J. Biochem. Biophys. Methods 70, 873 (2008) | (6) A. Ebner, et al.; Methods Mol. Biol. 418, 73 (2008) | (7) R. Mittal & M.P. Bruchez; Bioconjug. Chem. 22, 362 (2011)
Related Products
Product Name | Product Code | Supplier | 5-Aminofluorescein | CDX-A0018 | Chemodex | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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