ProSci

MIA40 Antibody

Product Code:
 
PSI-55-895
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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MIA40 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
MIA40 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-895-400ul400ul£626.00
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Further Information

Additional Names:
Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4, CHCHD4, MIA40
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).
Background References:
  • Daithankar, V.N., et al. Biochemistry 48(22):4828-4837(2009)
  • Chacinska, A., et al. J. Biol. Chem. 283(44):29723-29729(2008)
  • Terziyska, N., et al. FEBS Lett. 581(6):1098-1102(2007)
  • Hofmann, S., et al. J. Mol. Biol. 353(3):517-528(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the C-terminal region of human MIA40.
NCBI Gene ID #:
131474
NCBI Official Name:
Mitochondrial intermembrane space import and assembly protein 40
NCBI Official Symbol:
CHCHD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
Q8N4Q1
Protein GI Number:
62510498
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q8N4Q1
User NOte:
Optimal dilutions for each application to be determined by the researcher.