ProSci

B4GalT1 Antibody

Product Code:
 
PSI-63-690
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of B4GalT1 Antibody in HL-60 cell line lysates (35ug/lane)
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of B4GalT1 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-690-400ul400ul£626.00
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Further Information

Additional Names:
Beta-1,4-galactosyltransferase 1, Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1, 241-, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase, 241-, Processed beta-1,4-galactosyltransferase 1, B4GALT1, GGTB2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
B4GalT1 is an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing.
Background References:
  • Mengle-Gaw,L., et.al., Biochem. Biophys. Res. Commun. 176 (3), 1269-1276 (1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This B4GalT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-358 amino acids from the C-terminal region of human B4GalT1.
NCBI Gene ID #:
2683
NCBI Official Name:
Beta-1,4-galactosyltransferase 1
NCBI Official Symbol:
B4GALT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
P15291
Protein GI Number:
116241264
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P15291
User NOte:
Optimal dilutions for each application to be determined by the researcher.