ProSci

CD80 Antibody [12D9]

Product Code:
 
PSI-RF16046
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
12D9
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
CD80 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml
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Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
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Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL. <br><br>Red: CD80 Antibody [12D9] (RF16046) <br> Blue: DAPI staining
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Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL. <br><br>Red: CD80 Antibody [12D9] (RF16046) <br> Blue: DAPI staining
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Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL. <br><br>Red: CD80 Antibody [12D9] (RF16046) <br> Blue: DAPI staining
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Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.
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Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.
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Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.
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Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml
Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL. <br><br>Red: CD80 Antibody [12D9] (RF16046) <br> Blue: DAPI staining
Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL. <br><br>Red: CD80 Antibody [12D9] (RF16046) <br> Blue: DAPI staining
Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL. <br><br>Red: CD80 Antibody [12D9] (RF16046) <br> Blue: DAPI staining
Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.
Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.
Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.

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CodeSizePrice
PSI-RF16046-0.02mg0.02mg£150.00
Quantity:
PSI-RF16046-0.1mg0.1mg£515.00
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Further Information

Additional Names:
CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Application Note:
CD80 antibody can be used for detection of CD80 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Background:
CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Background References:
  • Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
  • Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.
Buffer:
CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CD80 antibody was raised against the extracellular domain of human CD80.
NCBI Gene ID #:
941
NCBI Official Name:
CD80 molecule
NCBI Official Symbol:
CD80
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 32 kDa

Observed: 50 kDa
Protein Accession #:
NP_005182
Protein GI Number:
4885123
Purification:
CD80 Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
P33681
User NOte:
Optimal dilutions for each application to be determined by the researcher.