ProSci

CD80 Antibody

Product Code:
 
PSI-61-757
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CD80 Antibody in HL-60 cell line lysates (35ug/lane)
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CD80 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-757-400ul400ul£626.00
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Further Information

Additional Names:
T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B71, B7, CD80, CD80, CD28LG, CD28LG1, LAB7
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CD80 is activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.
Background References:
  • Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
  • Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
  • Segat, L., et al. J. Gastroenterol. Hepatol. 24(12):1840-1846(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD80 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human CD80.
NCBI Gene ID #:
941
NCBI Official Name:
T-lymphocyte activation antigen CD80
NCBI Official Symbol:
CD80
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P33681
Protein GI Number:
461606
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P33681
User NOte:
Optimal dilutions for each application to be determined by the researcher.