ProSci

PCCB Antibody

Product Code:
 
PSI-63-665
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PCCB Antibody in Jurkat cell line lysates (35ug/lane)
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Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of PCCB Antibody in Jurkat cell line lysates (35ug/lane)
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-665-400ul400ul£626.00
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Further Information

Additional Names:
Propionyl-CoA carboxylase beta chain, mitochondrial, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
PCCB is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits.
Background References:
  • Yang,X., et.al.,Mol. Genet. Metab. 81 (4), 335-342 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This PCCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-107 amino acids from the Central region of human PCCB.
NCBI Gene ID #:
5096
NCBI Official Name:
Propionyl-CoA carboxylase beta chain, mitochondrial
NCBI Official Symbol:
PCCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
P05166
Protein GI Number:
124106304
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P05166
User NOte:
Optimal dilutions for each application to be determined by the researcher.