ProSci

ARHGDIA Antibody

Product Code:
 
PSI-61-586
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ARHGDIA Antibody in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane)
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Western blot analysis of ARHGDIA using rabbit polyclonal ARHGDIA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
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Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ARHGDIA Antibody in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane)
Western blot analysis of ARHGDIA using rabbit polyclonal ARHGDIA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-586-400ul400ul£626.00
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Further Information

Additional Names:
Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
ARHGDIA Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Background References:
  • Qiao,J., etc, Am. J. Physiol., Cell Physiol. 295 (5), C1161-C1168 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the C-terminal region of human ARHGDIA.
NCBI Gene ID #:
396
NCBI Official Name:
Rho GDP-dissociation inhibitor 1
NCBI Official Symbol:
ARHGDIA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
P52565
Protein GI Number:
1707892
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P52565
User NOte:
Optimal dilutions for each application to be determined by the researcher.