ProSci

PLCL2 Antibody

Product Code:
 
PSI-56-169
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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PLCL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
PLCL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-169-400ul400ul£626.00
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Further Information

Additional Names:
Inactive phospholipase C-like protein 2, PLC-L(2), PLC-L2, Phospholipase C-L2, Phospholipase C-epsilon-2, PLC-epsilon-2, PLCL2, KIAA1092, PLCE2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PLCL2 may play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity).
Background References:
  • Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009)
  • Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
  • Nakayama, M., et al. Genome Res. 12(11):1773-1784(2002)
  • Otsuki, M., et al. Biochem. Biophys. Res. Commun. 266(1):97-103(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1099-1126 amino acids from the C-terminal region of human PLCL2.
NCBI Gene ID #:
23228
NCBI Official Name:
Inactive phospholipase C-like protein 2
NCBI Official Symbol:
PLCL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
126 kDa
Protein Accession #:
Q9UPR0
Protein GI Number:
148880116
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9UPR0
User NOte:
Optimal dilutions for each application to be determined by the researcher.