Further Information
Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRYAB, CRYA2
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Crystallins are separated into two classes:
taxon-specific, or enzyme, and ubiquitous. The latter class
constitutes the major proteins of vertebrate eye lens and maintains
the transparency and refractive index of the lens. Since lens
central fiber cells lose their nuclei during development, these
crystallins are made and then retained throughout life, making them
extremely stable proteins. Mammalian lens crystallins are divided
into alpha, beta, and gamma families; beta and gamma crystallins
are also considered as a superfamily. Alpha and beta families are
further divided into acidic and basic groups. Seven protein regions
exist in crystallins: four homologous motifs, a connecting peptide,
and N- and C-terminal extensions. Alpha crystallins are composed of
two gene products: alpha-A and alpha-B, for acidic and basic,
respectively. Alpha crystallins can be induced by heat shock and
are members of the small heat shock protein (sHSP also known as the
HSP20) family. They act as molecular chaperones although they do
not renature proteins and release them in the fashion of a true
chaperone; instead they hold them in large soluble aggregates.
Post-translational modifications decrease the ability to chaperone.
These heterogeneous aggregates consist of 30-40 subunits; the
alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two
additional functions of alpha crystallins are an autokinase
activity and participation in the intracellular architecture.
Alpha-A and alpha-B gene products are differentially expressed;
alpha-A is preferentially restricted to the lens and alpha-B is
expressed widely in many tissues and organs. Elevated expression of
alpha-B crystallin occurs in many neurological diseases; a missense
mutation cosegregated in a family with a desmin-related myopathy.
- Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010)
- Jehle, S., et al. Nat. Struct. Mol. Biol. 17(9):1037-1042(2010)
- Kida, E., et al. J. Neuropathol. Exp. Neurol. 69(7):745-759(2010)
- Deng, Y., et al. BMB Rep 43(6):432-437(2010)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Pig, Rabbit, Rat
This CRYAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the Central region of human CRYAB.
1410
Alpha-crystallin B chain
CRYAB
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
P02511
117385
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Obesity,Neuroscience,Signal Transduction
P02511
Optimal dilutions for each application to be determined by the researcher.