ProSci

SPECC1 Antibody

Product Code:
 
PSI-56-244
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 5
Western blot analysis in HL-60 cell line lysates (35ug/lane).
2 / 5
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
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4 / 5
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
5 / 5
Confocal immunofluorescent analysis of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

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PSI-56-244-400ul400ul£626.00
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Further Information

Additional Names:
Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like, SPECC1-like protein, SPECC1L, CYTSA, KIAA0376
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
CYTSAis involved in cytokinesis and spindle organization.
Background References:
  • Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Zebrafish
Immunogen:
This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1.
NCBI Gene ID #:
23384
NCBI Official Name:
Cytospin-A
NCBI Official Symbol:
SPECC1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
125 kDa
Protein Accession #:
Q69YQ0
Protein GI Number:
300669640
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q69YQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.