ProSci

EN2 Antibody

Product Code:
 
PSI-62-925
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western Blot at 1:1000 dilution + Neuro-2a whole cell lysates Lysates/proteins at 20 ug per lane.
3 / 3
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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + Neuro-2a whole cell lysates Lysates/proteins at 20 ug per lane.

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PSI-62-925-400ul400ul£626.00
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Further Information

Additional Names:
Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.
Background References:
  • Poole S.J., Law M.L.Genomics 4:225-231(1989)
  • Benayed R., Gharani N.Am. J. Hum. Genet. 77:851-868(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Chicken, Xenopus
Immunogen:
This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
NCBI Gene ID #:
2020
NCBI Official Name:
Homeobox protein engrailed-2
NCBI Official Symbol:
EN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P19622
Protein GI Number:
21903415
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P19622
User NOte:
Optimal dilutions for each application to be determined by the researcher.