ProSci

ERO1LB Antibody

Product Code:
 
PSI-61-837
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane). ?
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ERO1LB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane). ?
ERO1LB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-837-400ul400ul£626.00
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Further Information

Additional Names:
ERO1-like protein beta, ERO1-L-beta, 184-, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta, ERO1LB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.
Background References:
  • Landa, I., et al. PLoS Genet.(9), E1000637 (2009) Savu, M.K., et al. Surg Obes Relat Dis 5(2):172-180(2009) Tavendale, R., et al. J. Allergy Clin. Immunol. 121(4):860-863(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ERO1LB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-293 amino acids from the C-terminal region of human ERO1LB.
NCBI Gene ID #:
56605
NCBI Official Name:
ERO1-like protein beta
NCBI Official Symbol:
ERO1LB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q86YB8
Protein GI Number:
116241353
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q86YB8
User NOte:
Optimal dilutions for each application to be determined by the researcher.