ProSci

Mouse Irak1 Antibody

Product Code:
 
PSI-57-735
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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PSI-57-735-400ul400ul£626.00
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Further Information

Additional Names:
Interleukin-1 receptor-associated kinase 1, IRAK, IRAK-1, Pelle-like protein kinase, mPLK, Irak1, Il1rak
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Irak1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The kinase activity of this enzyme may not be required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Background References:
  • Vaughan, T., et al. Mol. Immunol. 47(15):2515-2518(2010)
  • Hoshino, K., et al. J. Immunol. 184(7):3341-3345(2010)
  • Dong, J.W., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (3), H1079-H1087 (2010) :
  • Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Irak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-433 amino acids from the Central region of mouse Irak1.
NCBI Gene ID #:
16179
NCBI Official Name:
Interleukin-1 receptor-associated kinase 1
NCBI Official Symbol:
Irak1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
Q62406
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
Q62406
User NOte:
Optimal dilutions for each application to be determined by the researcher.