ProSci

TIGIT Antibody [4A11]

Product Code:
 
PSI-RF16055
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
4A11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
TIGIT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of TIGIT in over expressing HEK293 cells using RF16055 antibody at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml.
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Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
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Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.<br><br>Green: TIGIT Antibody [4A11] (RF16055) <br> Blue: DAPI staining
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Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.<br><br>Green: TIGIT Antibody [4A11] (RF16055) <br> Blue: DAPI staining
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Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.
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Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.
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Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with RF16051, RF16053, RF16054, RF16055, RF16056 and RF16058 antibodies at decreasing concentrations.
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A sandwich ELISA was performed using the anti-TIGIT mAb RF16055 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb RF16058-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
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Western blot analysis of TIGIT in over expressing HEK293 cells using RF16055 antibody at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml.
Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.<br><br>Green: TIGIT Antibody [4A11] (RF16055) <br> Blue: DAPI staining
Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.<br><br>Green: TIGIT Antibody [4A11] (RF16055) <br> Blue: DAPI staining
Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.
Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.
Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with RF16051, RF16053, RF16054, RF16055, RF16056 and RF16058 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-TIGIT mAb RF16055 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb RF16058-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.

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CodeSizePrice
PSI-RF16055-0.02mg0.02mg£150.00
Quantity:
PSI-RF16055-0.1mg0.1mg£515.00
Quantity:
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Further Information

Additional Names:
TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Application Note:
TIGIT antibody can be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 1 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 1 μg/mL. For Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
Background:
TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).
Background References:
  • Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
  • Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.
Buffer:
TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIGIT antibody was raised against the extracellular domain of human TIGIT
NCBI Gene ID #:
201633
NCBI Official Name:
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol:
TIGIT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26 kDa

Observed: 47 kDa
Protein Accession #:
NP_776160
Protein GI Number:
256600228
Purification:
TIGIT Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
Q495A1
User NOte:
Optimal dilutions for each application to be determined by the researcher.