Nordic MuBio

Collagen Type VI human

Product Code:
 
CO20611-0.1
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoblotting
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Radioimmunoassay (RIA)
  • Western Blot (WB)
Storage:
 
-20°C
1 / 1
❮❯

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CO20611-0.10.1 ml£480.00
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  • Further Information
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Further Information

Applications Description:
IHC(P), IFA, ELISA, RIA, IB/WB
Background:
Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100%, human collagen type I, II, III, IV and V <0.1%; human elastin and human laminin <0.1%; human fibronectin < 1% (RIA at 1:10.000 dilution).
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Concentration:
app. 1 mg/ml
Immunogen:
Purified collagen type VI from human placenta
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Human placenta
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method:
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Species Reactivity:
Human (cross-reactive with bovine, porcine, chicken, murine and rat tissue)
UniProt:
P12109 (CO6A1_HUMAN)
Working Concentration:
(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)

References

1. Ronzi?re M.C., Ricard-Blum S., Tiollier J., Hartmann D.J., Garrone R., Herbage D. (1990) Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic adult articular cartilage, with emphasis on type VI collagen. Biochim Biophys Acta. Apr 19;1038(2):222-30.
2. Silvester LM, Luck MR (1999) Distribution of extracellular matrix components in the developing ruminant corpus luteum: a wound repair hypothesis for luteinization. J Reprod Fertil. May;116(1):187-98.