NSJ Bioreagents

Actin Antibody / ACTA1

Product Code:
 
NSJ-R32166
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the anti-Actin antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 8
Western blot testing of 1) rat heart, 2) rat brain, 3) rat kidney, 4) mouse heart, 5) mouse brain, 6) mouse spleen and 7) mouse kidney tissue lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
2 / 8
Western blot testing of human 1) placenta, 2) HeLa, 3) HepG2, 4) 293T, 5) A431, 6) U-87 MG, 7) U937 and 8) K562 cell lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
3 / 8
IHC staining of FFPE human placental tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human colorectal adenocarcinoma tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human tonsil tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human breast cancer tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
IHC staining of FFPE mouse heart tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 8
IHC staining of FFPE rat heart tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
❮❯

Western blot testing of 1) rat heart, 2) rat brain, 3) rat kidney, 4) mouse heart, 5) mouse brain, 6) mouse spleen and 7) mouse kidney tissue lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
Western blot testing of human 1) placenta, 2) HeLa, 3) HepG2, 4) 293T, 5) A431, 6) U-87 MG, 7) U937 and 8) K562 cell lysate with anti-Actin antibody. Expected molecular weight: 42-45 kDa.
IHC staining of FFPE human placental tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with anti-Actin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R32166-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
Optimal dilution of the anti-Actin antibody should be determined by the researcher.
Description:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ITKQEYDEAGPSIVHRKCF of human ACTA1 were used as the immunogen for the anti-Actin antibody.
Limitation:
This anti-Actin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P68133