NSJ Bioreagents

GAD65 Antibody

Product Code:
 
NSJ-R30474
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GAD65 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of GAD65 antibody and rat brain tissue lysate~
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IHC-P: GAD65 antibody testing of rat brain tissue
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Western blot testing of GAD65 antibody and rat brain tissue lysate~
IHC-P: GAD65 antibody testing of rat brain tissue

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CodeSizePrice
NSJ-R30474-100ug100 ug£535.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the GAD65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutamate decarboxylase or glutamic acid decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD65 is the isoform with molecular weight of 65 kDa. Karlsen et al.(1991) mapped the GAD2 gene to chromosome 10p13-p11.2 by in situ hybridization. Bu et al.(1992) isolated a cDNA corresponding to the GAD2 gene from a human brain cDNA library. The deduced 585-amino acid protein has a molecular mass of 65 kD. GAD65 is a target of auto-antibodies in people who later develop type 1 diabetes mellitus or latent auto-immune diabetes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of the human protein (ENPGTARAWCQVAQKFT) was used as the immunogen for this GAD65 antibody (100% homologous in human, mouse and rat).
Limitation:
This GAD65 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q05329