NSJ Bioreagents

GNS Antibody / Glucosamine (N-acetyl)-6-sulfatase

Product Code:
 
NSJ-RQ4270
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GNS antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
Western blot testing of human 1) HepG2, 2) PANC-1 and 3) SGC-7901 cell lysate with GNS antibody at 0.5ug/ml. Predicted molecular weight ~62 kDa, can be observed at ~94 kDa.
2 / 2
Western blot testing of rat 1) lung, 2) kidney, 3) testis, 4) PC-12 lysate and mouse 5) lung, 6) kidney, 7) testis, 8) spleen and 9) thymus lysate with GNS antibody at 0.5ug/ml. Predicted molecular weight ~62 kDa, can be observed at ~94 kDa.
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Western blot testing of human 1) HepG2, 2) PANC-1 and 3) SGC-7901 cell lysate with GNS antibody at 0.5ug/ml. Predicted molecular weight ~62 kDa, can be observed at ~94 kDa.
Western blot testing of rat 1) lung, 2) kidney, 3) testis, 4) PC-12 lysate and mouse 5) lung, 6) kidney, 7) testis, 8) spleen and 9) thymus lysate with GNS antibody at 0.5ug/ml. Predicted molecular weight ~62 kDa, can be observed at ~94 kDa.

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CodeSizePrice
NSJ-RQ4270-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GNS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
N-acetylglucosamine-6-sulfatase, also known as glucosamine (N-acetyl)-6-sulfatase, is an enzyme that in humans is encoded by the GNS gene. The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids W238-R355 was used as the immunogen for the GNS antibody.
Limitation:
This GNS antibody is available for research use only.
Localization:
Lysosome
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15586