NSJ Bioreagents

MAVS Antibody (IPS-1)

Product Code:
 
NSJ-R32822
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MAVS antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 6
IHC testing of FFPE human liver cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 6
IHC testing of FFPE human intestinal cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 6
IHC testing of FFPE human lung cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 6
IHC testing of FFPE human breast cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 6
Western blot testing of human placenta tissue with MAVS antibody at 0.5ug/ml. Predicted molecular weight: 51-54 kDa (cleaved), 57 kDa (unmodified), 75 kDa (aggregated).
6 / 6
Flow cytometry testing of human A431 cells with MAVS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAVS antibody.
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IHC testing of FFPE human liver cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human intestinal cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human lung cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with MAVS antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of human placenta tissue with MAVS antibody at 0.5ug/ml. Predicted molecular weight: 51-54 kDa (cleaved), 57 kDa (unmodified), 75 kDa (aggregated).
Flow cytometry testing of human A431 cells with MAVS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAVS antibody.

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CodeSizePrice
NSJ-R32822-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MAVS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Mitochondrial antiviral-signaling protein (MAVS) is a protein that in humans is encoded by the MAVS gene. The protein is also known by the names VISA (virus-induced signaling adapter), IPS-1 and Cardif. This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids L34-Q96 was used as the immunogen for the MAVS antibody.
Limitation:
This MAVS antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q7Z434