NSJ Bioreagents

MDM2 Antibody

Product Code:
 
NSJ-R30449
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the MDM2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of MDM2 antibody and Lane 1: rat testis; 2: rat brain; 3: rat heart; 4: human SKOV-3; 5: COLO320; 6: HeLa; 7: mouse HEPA; 8: monkey COS7 cell lysate. Predicted/observed molecular weight: ~55 kDa.
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Western blot testing of MDM2 antibody and Lane 1: rat testis; 2: rat brain; 3: rat heart; 4: human SKOV-3; 5: COLO320; 6: HeLa; 7: mouse HEPA; 8: monkey COS7 cell lysate. Predicted/observed molecular weight: ~55 kDa.

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CodeSizePrice
NSJ-R30449-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MDM2 is an important negative regulator of the p53 tumor suppressor. The protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 343-358 (EKAKLENSTQAEEGFD) were used as the immunogen for this MDM2 antibody.
Limitation:
This MDM2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q00987